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Table 4 Examples of published liquid chromatography parameters for fatty acid analysis

From: Assessing diatom-mediated fatty acids in intertidal biofilm: a new conservation concern

  LC column Mobile phases Eluent gradients
Chu et al. (2009) ACQUITY UPLC BEH-C18, 130 Å, 1.7 µm, 2.1 mm × 50 mm, waters Solvent A: water
Solvent B: acetonitrile
70% A, 30% B decreasing to 25% A, 75% B over 10 min
Increasing to 100% B over 10 min
Samburova et al. (2013) ACQUITY UPLC BEH-C18, 130 Å, 1.7 µm, 2.1 mm × 50 mm, waters Solvent A: acetonitrile
Solvent B: 10 mM ammonium formate in methanol
20% A, 80% B for 5 min
Increasing to 100% B over 10 min
Held at 100% B for 45 min
Schlotterbeck et al. (2018) (untargeted analysis) Kinetex, 100 Å, 2.6 µm, 150 × 2.1 mm; C8, TMS end capped Solvent A: 10 mM aqueous ammonium acetate buffer
Solvent B: 55% ACN, 40% IPA and 5% 10 mM aqueous ammonium acetate buffer
60% A, 40% B, increasing to 100% B in 15 min
Held at 100% B for 5 min
Schlotterbeck et al. (2018) (targeted analysis of FA(16:4)n‐3) Kinetex, 100 Å, 2.6 µm, 150 × 2.1 mm; C8, TMS end capped Solvent A: 10 mM aqueous ammonium acetate buffer
Solvent B: 55% ACN, 40% IPA and 5% 10 mM aqueous ammonium acetate buffer
60% A, 40% B, increasing to 100% B in 5 min
Held at 100% for 2 min
  1. FA(16:4)n‐3: hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid