Table 4 Examples of published liquid chromatography parameters for fatty acid analysis
From: Assessing diatom-mediated fatty acids in intertidal biofilm: a new conservation concern
LC column | Mobile phases | Eluent gradients | |
|---|---|---|---|
Chu et al. (2009) | ACQUITY UPLC BEH-C18, 130 Å, 1.7 µm, 2.1 mm × 50 mm, waters | Solvent A: water Solvent B: acetonitrile | 70% A, 30% B decreasing to 25% A, 75% B over 10 min Increasing to 100% B over 10 min |
Samburova et al. (2013) | ACQUITY UPLC BEH-C18, 130 Å, 1.7 µm, 2.1 mm × 50 mm, waters | Solvent A: acetonitrile Solvent B: 10 mM ammonium formate in methanol | 20% A, 80% B for 5 min Increasing to 100% B over 10 min Held at 100% B for 45 min |
Schlotterbeck et al. (2018) (untargeted analysis) | Kinetex, 100 Å, 2.6 µm, 150 × 2.1 mm; C8, TMS end capped | Solvent A: 10 mM aqueous ammonium acetate buffer Solvent B: 55% ACN, 40% IPA and 5% 10 mM aqueous ammonium acetate buffer | 60% A, 40% B, increasing to 100% B in 15 min Held at 100% B for 5 min |
Schlotterbeck et al. (2018) (targeted analysis of FA(16:4)n‐3) | Kinetex, 100 Å, 2.6 µm, 150 × 2.1 mm; C8, TMS end capped | Solvent A: 10 mM aqueous ammonium acetate buffer Solvent B: 55% ACN, 40% IPA and 5% 10 mM aqueous ammonium acetate buffer | 60% A, 40% B, increasing to 100% B in 5 min Held at 100% for 2 min |